2Y9H
Structure A of CRISPR endoribonuclease Cse3 bound to 19 nt RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.860, 149.810, 87.260 |
| Unit cell angles | 90.00, 94.60, 90.00 |
Refinement procedure
| Resolution | 58.697 - 2.500 |
| R-factor | 0.232 |
| Rwork | 0.231 |
| R-free | 0.28950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y8w |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.215 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.700 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 0.780 |
| Number of reflections | 75718 | |
| <I/σ(I)> | 13.84 | 2.14 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 5.6 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 0.1 M MES, PH 6.2, 8% PEG (W/V) 10000. |
