2Y9G
High-resolution Structural Insights on the Sugar-recognition and Fusion Tag Properties of a Versatile b-Trefoil Lectin Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 32 |
Unit cell lengths | 62.150, 62.150, 37.650 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.970 - 1.670 |
R-factor | 0.178 |
Rwork | 0.175 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1w3a |
RMSD bond length | 0.027 |
RMSD bond angle | 2.249 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.000 | 1.760 |
High resolution limit [Å] | 1.670 | 1.670 |
Rmerge | 0.100 | 0.360 |
Number of reflections | 18678 | |
<I/σ(I)> | 12.6 | 5.2 |
Completeness [%] | 99.0 | 99 |
Redundancy | 6 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 12% (W/V) PEG 4000, 100 MM TRIS-HCL, PH 8.5, 150 MM SODIUM ACETATE |