2Y8W
Structure of CRISPR endoribonuclease Cse3 bound to 20 nt RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-20 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.100, 66.150, 74.150 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.362 - 1.800 |
R-factor | 0.1683 |
Rwork | 0.166 |
R-free | 0.21010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wj9 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.064 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.260 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.120 | 0.700 |
Number of reflections | 20275 | |
<I/σ(I)> | 14.51 | 3.57 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 7.4 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 0.1 M MES PH 6.5, 30% (V/V) PEG 300. |