2Y8V
Structure of chitinase, ChiC, from Aspergillus fumigatus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-22 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.137, 145.748, 82.899 |
| Unit cell angles | 90.00, 93.29, 90.00 |
Refinement procedure
| Resolution | 82.760 - 1.990 |
| R-factor | 0.16959 |
| Rwork | 0.169 |
| R-free | 0.21348 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.989 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.900 | 2.050 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.070 | 0.170 |
| Number of reflections | 76641 | |
| <I/σ(I)> | 63 | |
| Completeness [%] | 89.5 | 47.9 |
| Redundancy | 16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M BIS-TRIS PH 6.5, 28 % V/V PEG 2000 |
