2Y8N
Crystal structure of glycyl radical enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 132.280, 227.760, 148.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.184 - 1.750 |
| R-factor | 0.1865 |
| Rwork | 0.184 |
| R-free | 0.22780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r9d |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.085 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.180 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.090 | 0.399 |
| Number of reflections | 217979 | |
| <I/σ(I)> | 15.5 | 5.7 |
| Completeness [%] | 98.0 | 95 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 22 % PEG MME 550, 30 MM MGCL2, 100 MM TRIS/HCL PH 7.5 - 8.4 . |
