2Y8L
Structure of the regulatory fragment of mammalian aMPK in complex with two ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-23 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.567, 121.464, 125.922 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 87.420 - 2.500 |
R-factor | 0.229 |
Rwork | 0.228 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v8q |
RMSD bond length | 0.010 |
RMSD bond angle | 1.428 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.060 | 0.310 |
Number of reflections | 24672 | |
<I/σ(I)> | 23.1 | 3.5 |
Completeness [%] | 98.0 | 85.8 |
Redundancy | 5.9 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |