2Y8H
STRUCTURE OF THE FIRST GAF DOMAIN E87G MUTANT OF MYCOBACTERIUM TUBERCULOSIS DOSS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 294 |
| Detector technology | CCD |
| Collection date | 2009-10-13 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.740, 86.764, 100.459 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.240 - 1.900 |
| R-factor | 0.21226 |
| Rwork | 0.210 |
| R-free | 0.25585 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w3d |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.428 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 0.320 |
| Number of reflections | 25905 | |
| <I/σ(I)> | 24.8 | 4.4 |
| Completeness [%] | 99.0 | 86.2 |
| Redundancy | 6.8 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | PROTEIN WAS CRYSTALLIZED FROM 17% PEG 3350, 200 MM CALCIUM ACETATE, 100 MM TRIS-HCL, PH 7.0 |
