2Y8G
Structure of the Ran-binding domain from human RanBP3 (E352A-R353V double mutant)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 61.320, 61.320, 137.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.654 - 1.610 |
R-factor | 0.149 |
Rwork | 0.146 |
R-free | 0.19880 |
Structure solution method | SIRAS |
Starting model (for MR) | NONE |
RMSD bond length | 0.004 |
RMSD bond angle | 0.845 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELXD |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.700 |
High resolution limit [Å] | 1.610 | 1.610 |
Rmerge | 0.060 | 0.530 |
Number of reflections | 37445 | |
<I/σ(I)> | 26.7 | 4.3 |
Completeness [%] | 94.7 | 81.1 |
Redundancy | 10.7 | 9.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 1.5 M LI2SO4, 100 MM HEPES PH 7.5 |