2Y8G
Structure of the Ran-binding domain from human RanBP3 (E352A-R353V double mutant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 61.320, 61.320, 137.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.654 - 1.610 |
| R-factor | 0.149 |
| Rwork | 0.146 |
| R-free | 0.19880 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.845 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.700 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.060 | 0.530 |
| Number of reflections | 37445 | |
| <I/σ(I)> | 26.7 | 4.3 |
| Completeness [%] | 94.7 | 81.1 |
| Redundancy | 10.7 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 1.5 M LI2SO4, 100 MM HEPES PH 7.5 |
