2Y8D
STRUCTURE OF DBL6 EPSILON DOMAIN FROM VAR2CSA STRAIN FCR3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2010-05-22 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 85.859, 56.229, 75.849 |
| Unit cell angles | 90.00, 123.09, 90.00 |
Refinement procedure
| Resolution | 43.920 - 1.840 |
| R-factor | 0.2051 |
| Rwork | 0.203 |
| R-free | 0.23520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wau |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.9.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.200 | 1.870 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.060 | 1.000 |
| Number of reflections | 24727 | |
| <I/σ(I)> | 12.4 | 0.6 |
| Completeness [%] | 93.6 | 73.7 |
| Redundancy | 3.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 30% PEG 4000, 0.1M NA ACETATE, PH 4.8, 0.1M MGCL2, 2.6% BENZAMIDINE, 10MG/ML PROTEIN IN 0.02M TRIS PH 8, 0.14M NACL, 0.045M CYSTAMINE; VAPOUR DIFFUSION |
