2Y8C
Plasmodium falciparum dUTPase in complex with a trityl ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 41 |
| Unit cell lengths | 77.160, 77.160, 110.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.900 - 2.100 |
| R-factor | 0.1905 |
| Rwork | 0.188 |
| R-free | 0.24048 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vyq |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.258 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.900 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.070 | 0.530 |
| Number of reflections | 35984 | |
| <I/σ(I)> | 13 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 3.3M AMMONIUM SULPHATE, 0.1M BIS-TRIS PH 6.0 |
