2Y7W
DntR Inducer Binding Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.187, 118.957, 80.558 |
| Unit cell angles | 90.00, 108.63, 90.00 |
Refinement procedure
| Resolution | 47.140 - 2.890 |
| R-factor | 0.244 |
| Rwork | 0.242 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1utb MOLECULE A |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.409 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.4_94)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.100 | 3.040 |
| High resolution limit [Å] | 2.900 | 2.890 |
| Rmerge | 0.170 | 0.580 |
| Number of reflections | 20475 | |
| <I/σ(I)> | 5.4 | 1.7 |
| Completeness [%] | 94.1 | 88.8 |
| Redundancy | 2.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M BIS-TRIS PH 6.5, 0.2M MGCL2, 25% PEG 3350 |
