2Y7K
DntR Inducer Binding Domain in Complex with Salicylate. Monoclinic crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.905, 82.106, 86.273 |
| Unit cell angles | 90.00, 111.33, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.950 |
| R-factor | 0.20252 |
| Rwork | 0.200 |
| R-free | 0.25002 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.598 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.100 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.860 |
| Number of reflections | 65440 | |
| <I/σ(I)> | 10.8 | 2.2 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 31% PEG 8000, 0.1 M TRIS HCL PH 8.0, AND 0.2 M NA/K TARTRATE. |
