2Y7I
Structural basis for high arginine specificity in Salmonella typhimurium periplasmic binding protein STM4351.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 273 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.317, 56.331, 88.370 |
| Unit cell angles | 90.00, 100.17, 90.00 |
Refinement procedure
| Resolution | 47.280 - 1.900 |
| R-factor | 0.19949 |
| Rwork | 0.198 |
| R-free | 0.22602 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lah |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.084 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.280 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.880 |
| Rmerge | 0.100 | 0.860 |
| Number of reflections | 35156 | |
| <I/σ(I)> | 17.9 | 2.4 |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 6 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 18% PEG 8000, 100MM SODIUM CACODYLATE, 200MM ZINC ACETATE., pH 7.4 |
