2Y7E
Crystal structure of the 3-keto-5-aminohexanoate cleavage enzyme (Kce) from Candidatus Cloacamonas acidaminovorans (tetragonal form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2010-05-22 |
Detector | ADSC CCD |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 102.188, 102.188, 101.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.760 - 1.280 |
R-factor | 0.14465 |
Rwork | 0.143 |
R-free | 0.17020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y7d |
RMSD bond length | 0.012 |
RMSD bond angle | 1.467 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0093) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.350 |
High resolution limit [Å] | 1.280 | 1.280 |
Rmerge | 0.080 | 0.470 |
Number of reflections | 133532 | |
<I/σ(I)> | 12.4 | 3.3 |
Completeness [%] | 97.6 | 89.6 |
Redundancy | 7 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 20% PEG3350, 0.220 M MG FORMATE, 26.4% GLYCEROL, pH 7.0 |