2Y78
Crystal structure of BPSS1823, a Mip-like chaperone from Burkholderia pseudomallei
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-23 |
Detector | ADSC CCD |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 54.677, 54.677, 119.172 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.646 - 0.910 |
R-factor | 0.1097 |
Rwork | 0.109 |
R-free | 0.11610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1FKB AND 1ROT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.586 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.300 | 0.960 |
High resolution limit [Å] | 0.910 | 0.910 |
Rmerge | 0.080 | 0.400 |
Number of reflections | 125244 | |
<I/σ(I)> | 13.8 | 2.2 |
Completeness [%] | 98.3 | 88.2 |
Redundancy | 5.9 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 1.1 M (NH4)2SO4, 0.05 M BIS-TRIS PH 5.5 |