2Y6X
Structure of Psb27 from Thermosynechococcus elongatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-30 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.930, 50.820, 53.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.310 - 1.600 |
| R-factor | 0.19246 |
| Rwork | 0.190 |
| R-free | 0.23775 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2kmf |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.892 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0104) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.310 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.070 | 0.530 |
| Number of reflections | 13700 | |
| <I/σ(I)> | 8.31 | 3.16 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.52 | 6.61 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 35% W/V PEG 4K MIXED WITH EQUAL VOLUME OF 20MG/ML PROTEIN SOLUTION, HANGING DROP VAPOUR DIFFUSION |
