2Y6Q
Structure of the TetX monooxygenase in complex with the substrate 7- Iodtetracycline
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.3 |
Synchrotron site | BESSY |
Beamline | 14.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-23 |
Detector | MARRESEARCH SX-165 |
Spacegroup name | P 1 |
Unit cell lengths | 68.700, 79.960, 88.000 |
Unit cell angles | 111.10, 90.41, 92.96 |
Refinement procedure
Resolution | 51.980 - 2.370 |
R-factor | 0.21937 |
Rwork | 0.218 |
R-free | 0.23776 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xdo |
RMSD bond length | 0.020 |
RMSD bond angle | 1.825 |
Data reduction software | XDS |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.980 | 2.500 |
High resolution limit [Å] | 2.370 | 2.370 |
Rmerge | 0.090 | 0.490 |
Number of reflections | 66166 | |
<I/σ(I)> | 7.3 | 2.1 |
Completeness [%] | 93.5 | 69.6 |
Redundancy | 2.6 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | pH 7 |