2Y6N
STRUCTURE OF LINEAR GRAMICIDIN D OBTAINED USING TYPE I CRYSTALS GROWN IN A 8.8 MONOACYLGLYCEROL LIPID CUBIC PHASE.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-22 |
| Detector | MARRESEARCH MX-300 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.616, 62.783, 30.602 |
| Unit cell angles | 90.00, 100.02, 90.00 |
Refinement procedure
| Resolution | 30.150 - 1.260 |
| R-factor | 0.14917 |
| Rwork | 0.148 |
| R-free | 0.16983 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y5m |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.775 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.150 | 1.290 |
| High resolution limit [Å] | 1.260 | 1.260 |
| Rmerge | 0.060 | 0.280 |
| Number of reflections | 28320 | |
| <I/σ(I)> | 22.6 | 3.5 |
| Completeness [%] | 96.4 | 76.9 |
| Redundancy | 7.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 30 %(W/V) POLYETHYLENE GLYCOL (PEG) 8000, 0.2 M NH4SO4, LIPIDIC CUBIC PHASE OF 8.8 MAG (SN-1-O-(CIS-8)HEXADECENYLGLYCEROL) WAS USED IN A RATIO OF 1:20 (MOL/MOL) GRAMICIDIN D TO 8.8 MAG. |
