2Y6H
X-2 L110F CBM4-2 Carbohydrate Binding Module from a Thermostable Rhodothermus marinus Xylanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-10 |
| Detector | MARRESEARCH SX-165 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.510, 50.350, 62.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.080 |
| R-factor | 0.137 |
| R-free | 0.17000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | XYLOPENTOSE BOUND TO X-2 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.030 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.110 |
| High resolution limit [Å] | 1.080 | 1.080 |
| Rmerge | 0.030 | 0.320 |
| Number of reflections | 62650 | |
| <I/σ(I)> | 18.8 | 3.5 |
| Completeness [%] | 96.1 | 90.1 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.1 M MIB BUFFER PH 6.0 25 % W/V PEG 1500 |
