2Y6E
Structure of the D1D2 domain of USP4, the conserved catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-06 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 110.500, 151.030, 178.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.590 - 2.400 |
| R-factor | 0.1797 |
| Rwork | 0.178 |
| R-free | 0.21040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gfo |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.020 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.600 | 44.600 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.090 | 0.660 |
| Number of reflections | 111095 | |
| <I/σ(I)> | 11 | 1.4 |
| Completeness [%] | 94.9 | 74 |
| Redundancy | 3.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | PROTEIN WAS CRYSTALLIZED FROM 100MM BIS-TRIS PROPANE (PH8.5), 25MM NA2SO4, 18% PEG3350, THEN SOAKED IN 25% ETHYLENEGLYCOL |
