2Y65
Crystal structure of Drosophila melanogaster kinesin-1 motor domain dimer-tail complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2010-10-16 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 113.765, 190.714, 145.980 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.200 |
R-factor | 0.19787 |
Rwork | 0.195 |
R-free | 0.25192 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y5w |
RMSD bond length | 0.012 |
RMSD bond angle | 1.785 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.060 | 0.370 |
Number of reflections | 80517 | |
<I/σ(I)> | 21.7 | 5.5 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 8.8 | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.4 | 18% POLYETHYLENE GLYCOL-3350, 0.2M POTASSIUM CHLORIDE, 0.1M HEPES SODIUM PH 7.4 |