2Y5M
STRUCTURE OF LINEAR GRAMICIDIN D OBTAINED USING TYPE I CRYSTALS GROWN IN A 7.7 MONOACYLGLYCEROL LIPID CUBIC PHASE.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-05 |
| Detector | MARRESEARCH MX-300 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.639, 62.794, 30.664 |
| Unit cell angles | 90.00, 100.02, 90.00 |
Refinement procedure
| Resolution | 30.200 - 1.080 |
| R-factor | 0.13167 |
| Rwork | 0.130 |
| R-free | 0.15461 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1al4 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.704 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.200 | 1.100 |
| High resolution limit [Å] | 1.080 | 1.080 |
| Rmerge | 0.100 | 0.420 |
| Number of reflections | 46435 | |
| <I/σ(I)> | 15.8 | 2.4 |
| Completeness [%] | 94.9 | 65 |
| Redundancy | 6.3 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5.5 | 25 %(W/V) PEG 3350, 0.2 M LISO4, 0.1 M BIS TRIS AT PH 5.5, LIPIDIC CUBIC PHASE OF 7.7 MAG (SN-1-O-(CIS-7)TETRADECENYLGLYCEROL) WAS USED IN A RATIO OF 1:20 (MOL/MOL) GRAMICIDIN D TO LIPID. |
