2Y5C
Structure of human ferredoxin 2 (Fdx2)in complex with 2Fe2S cluster
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-19 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.380, 35.450, 75.510 |
| Unit cell angles | 90.00, 105.13, 90.00 |
Refinement procedure
| Resolution | 49.600 - 1.700 |
| R-factor | 0.17356 |
| Rwork | 0.173 |
| R-free | 0.20051 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.532 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXCDE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.600 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.060 | 0.460 |
| Number of reflections | 27945 | |
| <I/σ(I)> | 22 | 5.2 |
| Completeness [%] | 97.9 | 96.5 |
| Redundancy | 7.1 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.6 M SODIUM CITRATE |
