2Y53
Crystal structure of E257Q mutant of the box pathway encoded ALDH from Burkholderia xenovorans LB400
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 1 |
Unit cell lengths | 58.240, 67.810, 77.510 |
Unit cell angles | 111.22, 90.45, 113.51 |
Refinement procedure
Resolution | 23.370 - 1.400 |
R-factor | 0.15768 |
Rwork | 0.156 |
R-free | 0.18545 |
Structure solution method | OTHER |
RMSD bond length | 0.030 |
RMSD bond angle | 2.615 |
Data scaling software | CCP4 |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 23.440 |
High resolution limit [Å] | 1.400 |
Rmerge | 0.060 |
Number of reflections | 187905 |
<I/σ(I)> | 14.7 |
Completeness [%] | 95.6 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |