2Y51
Crystal structure of E167A mutant of the box pathway encoded ALDH from Burkholderia xenovorans LB400
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 1 |
Unit cell lengths | 57.590, 67.630, 77.270 |
Unit cell angles | 112.48, 90.14, 111.51 |
Refinement procedure
Resolution | 37.720 - 1.600 |
R-factor | 0.18171 |
Rwork | 0.179 |
R-free | 0.23029 |
Structure solution method | OTHER |
Starting model (for MR) | NONE |
RMSD bond length | 0.026 |
RMSD bond angle | 2.009 |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 39.250 |
High resolution limit [Å] | 1.600 |
Rmerge | 0.070 |
Number of reflections | 121799 |
<I/σ(I)> | 12.3 |
Completeness [%] | 93.4 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |