2Y4Z
Structure of the amino-terminal capsid restriction escape mutation N- MLV L10W
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.880, 33.647, 59.320 |
| Unit cell angles | 90.00, 111.91, 90.00 |
Refinement procedure
| Resolution | 24.681 - 2.001 |
| R-factor | 0.1774 |
| Rwork | 0.174 |
| R-free | 0.24380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1U7K (CHAIN A) |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.977 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.040 | 0.180 |
| Number of reflections | 9015 | |
| <I/σ(I)> | 25.1 | 6.3 |
| Completeness [%] | 99.6 | 97 |
| Redundancy | 3.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
