2Y4X
Structure of a domain from the type IV pilus biogenesis lipoprotein PilP, from Pseudomonas aeruginosa PA01
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU R-AXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 24.240, 87.790, 42.210 |
| Unit cell angles | 90.00, 103.77, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.233 |
| Rwork | 0.230 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y4y |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.321 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.060 | 0.380 |
| Number of reflections | 16259 | |
| <I/σ(I)> | 9.7 | 2.4 |
| Completeness [%] | 86.7 | 85.8 |
| Redundancy | 2.8 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M HEPES PH 7.5, 8% (V/V) ETHYLENE GLYCOL, 20% (W/V) PEG 10,000 |
