2Y4T
Crystal structure of the human co-chaperone P58(IPK)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | H 3 2 |
Unit cell lengths | 212.250, 212.250, 283.460 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 3.000 |
R-factor | 0.24582 |
Rwork | 0.243 |
R-free | 0.29058 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.043 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0081) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 3.080 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.080 | 0.560 |
Number of reflections | 44987 | |
<I/σ(I)> | 11.5 | 2.2 |
Completeness [%] | 96.6 | 79.2 |
Redundancy | 4.3 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1 M HEPES, PH 8.2, 1.4 M SUCCINIC ACID, 1% (W/V) PEG MME 2000 |