2Y4K

MANNOSYLGLYCERATE SYNTHASE IN COMPLEX WITH MG-GDP

Replaces:  2XW3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-28
Detector	ADSC QUANTUM 315r
Spacegroup name	P 32 2 1
Unit cell lengths	149.646, 149.646, 154.112
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.760 - 2.450
R-factor	0.20207
Rwork	0.201
R-free	0.22479
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bo7
RMSD bond length	0.015
RMSD bond angle	1.520
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	129.000	2.540
High resolution limit [Å]	2.450	2.450
Rmerge	0.070	0.520
Number of reflections	70281
<I/σ(I)>	17.5	2.9
Completeness [%]	100.0	100
Redundancy	6.1	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5		4% V/V ETHYLENE GLYCOL, 0.1 M BIS-TRIS PROPANE PH 5.5, 0.4 M MAGNESIUM FORMATE.