Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-09 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 148.975, 148.975, 154.973 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 129.020 - 2.300 |
| R-factor | 0.19934 |
| Rwork | 0.198 |
| R-free | 0.22163 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bo7 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.496 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | 0.370 |
| Number of reflections | 83970 | |
| <I/σ(I)> | 7.6 | 2.4 |
| Completeness [%] | 99.7 | 96.8 |
| Redundancy | 2.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 0.3 M SODIUM D-LACTATE, 0.1 M SODIUM ACETATE TRIHYDRATE, PH 4.6, 40% (V/V) 2-METHYL-1, 3-PROPANEDIOL |
