2Y4G
Structure of the Tirandamycin-bound FAD-dependent tirandamycin oxidase TamL in P212121 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2010-12-04 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.020, 130.400, 134.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 134.540 - 2.030 |
R-factor | 0.16271 |
Rwork | 0.159 |
R-free | 0.23566 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y08 |
RMSD bond length | 0.020 |
RMSD bond angle | 1.894 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 134.540 | 2.140 |
High resolution limit [Å] | 2.030 | 2.030 |
Rmerge | 0.120 | 0.680 |
Number of reflections | 67342 | |
<I/σ(I)> | 7.7 | 1.5 |
Completeness [%] | 91.7 | 63 |
Redundancy | 4.4 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 15% PEG 4000, 0.1M MGCL2, 0.1M HEPES 7.5, ADDITIVE 0.1M MGCL2. |