2Y3M
Structure of the extra-membranous domain of the secretin HofQ from Actinobacillus actinomycetemcomitans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 62 |
| Unit cell lengths | 119.610, 119.610, 51.280 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.000 - 2.300 |
| R-factor | 0.2063 |
| Rwork | 0.205 |
| R-free | 0.23669 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.044 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.030 | 0.610 |
| Number of reflections | 18861 | |
| <I/σ(I)> | 16.6 | 2.3 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 7.3 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2 M SODIUM FORMATE, 0.1 M SODIUM ACETATE PH 4.6 . |
