2Y3H
E63Q mutant of Cupriavidus metallidurans CH34 CnrXs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-06 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.968, 79.341, 93.040 |
| Unit cell angles | 90.00, 90.11, 90.00 |
Refinement procedure
| Resolution | 36.492 - 1.892 |
| R-factor | 0.1745 |
| Rwork | 0.172 |
| R-free | 0.21960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y3g |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.894 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.520 | 1.990 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.060 | 0.320 |
| Number of reflections | 35627 | |
| <I/σ(I)> | 8.6 | 2.5 |
| Completeness [%] | 96.2 | 91.8 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 16% PEG 2000MME, 15% GLYCEROL, pH 8 |
