2Y3H
E63Q mutant of Cupriavidus metallidurans CH34 CnrXs
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-06 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.968, 79.341, 93.040 |
Unit cell angles | 90.00, 90.11, 90.00 |
Refinement procedure
Resolution | 36.492 - 1.892 |
R-factor | 0.1745 |
Rwork | 0.172 |
R-free | 0.21960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y3g |
RMSD bond length | 0.006 |
RMSD bond angle | 0.894 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.520 | 1.990 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.060 | 0.320 |
Number of reflections | 35627 | |
<I/σ(I)> | 8.6 | 2.5 |
Completeness [%] | 96.2 | 91.8 |
Redundancy | 4.2 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 16% PEG 2000MME, 15% GLYCEROL, pH 8 |