2Y3B
Co-bound form of Cupriavidus metallidurans CH34 CnrXs
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-18 |
Detector | ADSC CCD |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 33.033, 33.033, 196.226 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.575 - 1.554 |
R-factor | 0.1776 |
Rwork | 0.176 |
R-free | 0.20380 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.017 |
RMSD bond angle | 1.583 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.040 | 1.640 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.050 | 0.330 |
Number of reflections | 16626 | |
<I/σ(I)> | 12.2 | 2.4 |
Completeness [%] | 99.1 | 95.2 |
Redundancy | 7.2 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 0.1M TRIS PH8, 1.6M AMMONIUM SULFATE, 15% GLYCEROL. |