2Y2C
crystal structure of AmpD Apoenzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-13 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 32 |
| Unit cell lengths | 68.080, 68.080, 93.080 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.530 - 1.802 |
| R-factor | 0.192 |
| Rwork | 0.188 |
| R-free | 0.24630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y28 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.049 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.400 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.130 | 0.940 |
| Number of reflections | 44489 | |
| <I/σ(I)> | 14 | 2 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 5.7 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.1 M TRIS PH 6.0, 0.1 M LI2SO4, 28% PEG 3350 |
