2Y2C
crystal structure of AmpD Apoenzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-13 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 32 |
Unit cell lengths | 68.080, 68.080, 93.080 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.530 - 1.802 |
R-factor | 0.192 |
Rwork | 0.188 |
R-free | 0.24630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y28 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.049 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.400 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.130 | 0.940 |
Number of reflections | 44489 | |
<I/σ(I)> | 14 | 2 |
Completeness [%] | 99.0 | 99 |
Redundancy | 5.7 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 0.1 M TRIS PH 6.0, 0.1 M LI2SO4, 28% PEG 3350 |