2Y24

STRUCTURAL BASIS FOR SUBSTRATE RECOGNITION BY ERWINIA CHRYSANTHEMI GH5 GLUCURONOXYLANASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-25
Detector	MARRESEARCH SX-165
Spacegroup name	P 32 2 1
Unit cell lengths	59.578, 59.578, 168.296
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.370 - 1.390
R-factor	0.12206
Rwork	0.120
R-free	0.16930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nof
RMSD bond length	0.024
RMSD bond angle	1.973
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.400
High resolution limit [Å]	1.390	1.390
Rmerge	0.080	0.420
Number of reflections	70207
<I/σ(I)>	8.5	1.3
Completeness [%]	98.6	98.6
Redundancy	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		CO-CRYSTALLIZATION USING 0.1 M IMIDAZOLE - DL-MALIC ACID BUFFER, PH 7.5 AND 20% PEG 1500 AS PRECIPITANT SOLUTION