2Y23
CRYSTAL STRUCTURE OF THE MYOMESIN DOMAINS MY9-MY11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.471, 41.487, 84.862 |
| Unit cell angles | 90.00, 88.39, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.500 |
| R-factor | 0.21127 |
| Rwork | 0.209 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MYOMESIN DOMAINS MY10-MY11 PDB ENTRY 2R15 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.577 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.530 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.110 | 0.520 |
| Number of reflections | 15174 | |
| <I/σ(I)> | 10.6 | 1.8 |
| Completeness [%] | 99.0 | 89.6 |
| Redundancy | 3.3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.18M MAGNESIUM ACETATE 20% W/V POLYETHYLENE GLYCOL 3350, pH 7.5 |
