2Y20
The mechanisms of HAMP-mediated signaling in transmembrane receptors - the A291I mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.490, 64.760, 93.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.810 - 1.650 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.087 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.700 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.090 | 0.550 |
| Number of reflections | 33647 | |
| <I/σ(I)> | 22.7 | 2.7 |
| Completeness [%] | 99.1 | 99.3 |
| Redundancy | 6.2 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 20 MM MOPS, 100 MM NACL, 0.1 M ZN ACETATE, 12% PEG 4000, PH 4.6. |
