2Y1R

Structure of MecA121 & ClpC N-domain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-07-27
Detector	RIGAKU HTC
Spacegroup name	P 21 21 21
Unit cell lengths	110.020, 124.720, 149.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.928 - 2.595
R-factor	0.2157
Rwork	0.213
R-free	0.25820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 3JTP 2y1q
RMSD bond length	0.051
RMSD bond angle	1.480
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.590	2.590
Rmerge	0.100	0.570
Number of reflections	60699
<I/σ(I)>	9.17	1.91
Completeness [%]	94.1	79.6
Redundancy	3.5	2.79

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				20%(W/V) POLYETHYLENE GLYCOL 3350, 300MM AMMONIUM TARTRATE AND 6MM N-NONYL-BETA-D-MALTOPYRANOSIDE