2Y1P
Catalytic domain of mouse 2',3'-cyclic nucleotide 3'- phosphodiesterase, complexed with citrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-14 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.950, 46.920, 54.850 |
Unit cell angles | 90.00, 95.51, 90.00 |
Refinement procedure
Resolution | 26.283 - 1.820 |
R-factor | 0.16 |
Rwork | 0.158 |
R-free | 0.19910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xmi |
RMSD bond length | 0.009 |
RMSD bond angle | 1.126 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.280 | 1.870 |
High resolution limit [Å] | 1.820 | 1.820 |
Rmerge | 0.070 | 0.680 |
Number of reflections | 19148 | |
<I/σ(I)> | 14.19 | 2.02 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 3.74 | 3.69 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 3.3 | pH 3.3 |