2Y1B
Crystal structure of the E. coli outer membrane lipoprotein RcsF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X12 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-07 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97791, 1.5 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 55.714, 55.714, 61.679 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.250 - 2.000 |
R-factor | 0.19913 |
Rwork | 0.196 |
R-free | 0.25752 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.022 |
RMSD bond angle | 2.157 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | Auto-Rickshaw |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.000 | 2.200 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.080 | 0.530 |
Number of reflections | 7786 | |
<I/σ(I)> | 11.5 | 2.7 |
Completeness [%] | 99.6 | 99.6 |
Redundancy | 3.5 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | HANGING DROP: PROTEIN 11MG/ML MIXED WITH I3C 20 MM RESERVOIR: 1.8M AMMONIUM SULFATE, 0.1M SODIUM ACETATE PH 4.5, DROP: 1 MICROL AND 1 MICROL. |