2Y0T
The mechanisms of HAMP-mediated signaling in transmembrane receptors - the A291F mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 88.070, 34.320, 47.650 |
Unit cell angles | 90.00, 117.79, 90.00 |
Refinement procedure
Resolution | 19.560 - 1.300 |
R-factor | 0.17491 |
Rwork | 0.173 |
R-free | 0.20855 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | NONE |
RMSD bond length | 0.032 |
RMSD bond angle | 2.762 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.330 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.030 | 0.300 |
Number of reflections | 28953 | |
<I/σ(I)> | 19.6 | 3.6 |
Completeness [%] | 94.7 | 96.5 |
Redundancy | 3.05 | 3.08 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 20 MM MOPS, 100 MM NACL, 20% PEG 3350., pH 6.5 |