2Y0Q
The mechanisms of HAMP-mediated signaling in transmembrane receptors - the A291C mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 40.190, 55.450, 158.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.000 |
R-factor | 0.21818 |
Rwork | 0.215 |
R-free | 0.26939 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.314 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 2.050 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.050 | 0.490 |
Number of reflections | 10902 | |
<I/σ(I)> | 15.3 | 2.1 |
Completeness [%] | 97.2 | 97 |
Redundancy | 4.7 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6 | pH 5.6 |