2Y09
The cyanobacterial PP2C-like phosphatase tPphA requires three metals in the catalytic center for efficient catalysis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 38.714, 151.678, 82.777 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.330 - 1.700 |
R-factor | 0.1936 |
Rwork | 0.192 |
R-free | 0.21850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2j82 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.127 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.330 | 1.800 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.040 | 0.400 |
Number of reflections | 27138 | |
<I/σ(I)> | 19.2 | 2.67 |
Completeness [%] | 99.1 | 97 |
Redundancy | 1 | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 30% PEG3350 0.2M CACL2 0.1M TRIS-HCL PH 8.0 |