2XZF

CRYSTAL STRUCTURE OF A COMPLEX BETWEEN THE WILD-TYPE LACTOCOCCUS LACTIS FPG (MUTM) AND AN OXIDIZED PYRIMIDINE CONTAINING DNA AT 293K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-21
Detector	ADSC CCD
Spacegroup name	P 41 21 2
Unit cell lengths	91.569, 91.569, 142.777
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.960 - 1.799
R-factor	0.1611
Rwork	0.160
R-free	0.19110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xc8
RMSD bond length	0.008
RMSD bond angle	1.254
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.060	0.510
Number of reflections	56390
<I/σ(I)>	25.3	4.5
Completeness [%]	99.1	96.5
Redundancy	9.2	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.6		HEPES, SODIUM CITRATE, PH 7.0