2XZ3
BLV TM hairpin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | H 3 |
| Unit cell lengths | 107.595, 107.595, 119.170 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.950 |
| R-factor | 0.19445 |
| Rwork | 0.192 |
| R-free | 0.23693 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mg1 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.383 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.040 | 0.570 |
| Number of reflections | 37151 | |
| <I/σ(I)> | 23.9 | 2.1 |
| Completeness [%] | 99.0 | 90 |
| Redundancy | 3.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 24.5%(V/V) ISOPROPANOL, 13.5%(V/V) PEG 4000, 0.1M SODIUM CITRATE, PH 5.6 |
