2XZ2
Crystal structure of CstF-50 homodimerization domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 46.230, 52.230, 57.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.130 - 1.400 |
| R-factor | 0.2012 |
| Rwork | 0.199 |
| R-free | 0.24520 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXCD |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.040 | 0.280 |
| Number of reflections | 13968 | |
| <I/σ(I)> | 21.56 | 5.13 |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 3.52 | 5.19 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100 MM HEPES PH 7.5, 3% PEG400, 1.4 M AMMONIUM SULFATE, 15% GLYCEROL |
