2XYR
Crystal structure of the nsp16 nsp10 SARS coronavirus complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-21 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 67.974, 184.107, 129.206 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.930 - 2.500 |
| R-factor | 0.2026 |
| Rwork | 0.201 |
| R-free | 0.23517 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fyg |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.254 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.580 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 0.400 |
| Number of reflections | 28921 | |
| <I/σ(I)> | 12.5 | 4.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.3 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 0.1M CHES, 1.52M MGCL2, pH 9 |
