2XXP
A widespread family of bacterial cell wall assembly proteins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-01-25 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 73.560, 73.560, 163.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.681 - 1.692 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.22410 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.310 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.880 | 1.780 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.060 | 0.400 |
| Number of reflections | 50786 | |
| <I/σ(I)> | 17.4 | 3.9 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 6.8 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2 M DI-AMMONIUM HYDROGEN CITRATE PH 5.0, 15 % W/V PEG3350 |
