2XWX
Vibrio cholerae colonization factor GbpA crystal structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-20 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.955, 120.182, 67.177 |
Unit cell angles | 90.00, 108.41, 90.00 |
Refinement procedure
Resolution | 19.940 - 1.800 |
R-factor | 0.207 |
Rwork | 0.207 |
R-free | 0.24600 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.008 |
RMSD bond angle | 1.124 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | PHENIX ((PHENIX.REFINE: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.050 | 0.330 |
Number of reflections | 73728 | |
<I/σ(I)> | 14.4 | 3 |
Completeness [%] | 96.1 | 91.6 |
Redundancy | 3 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.2 M MG(HCO3)2, 50% (W/V) PEG 3350, 3.33% (W/V) D-SORBITOL, PH 7.5 |